| Gene | Defb45 |
| Chromosome | 2 |
| Deletion Start | 152,596,390 |
| Deletion End | 152,593,222 |
| Deletion Size | 3,169 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 152,596,307 | 152,596,327 | TCCTTCAGAGGAGGGACAGAG |
| TUP | 152,596,280 | 152,596,305 | TGGGTCTAGTCAGGGTACTTATGGCC |
| TUR | 152,596,242 | 152,596,261 | GCCCAGACAGTGTCTCCTAC |
| TDF | 152,593,658 | 152,593,682 | TTCAAACCCAATTCTGTAGATCCTG |
| TDP | 152,593,626 | 152,593,653 | TTCATGTAACCCTCATAACCCACTGTGC |
| TDR | 152,593,573 | 152,593,597 | AGCCCTTCATAAACTCCACCTCTTG |
PCR Primers
| Primer | Sequence |
| SU | GAGTAGAGGATTCTGGGCTGG |
| SD | ACACACTTTGGCATTTCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16077A-B3 | VGB6 | C57BL/6NTac | |
| 16077A-C9 | VGB6 | C57BL/6NTac | |
| 16077A-D1 | VGB6 | C57BL/6NTac | |
| 16077A-D5 | VGB6 | C57BL/6NTac | |
| 16077A-D7 | VGB6 | C57BL/6NTac | |
| 16077A-E9 | VGB6 | C57BL/6NTac | |
