| Gene | Actr5 |
| Chromosome | 2 |
| Deletion Start | 158,638,904 |
| Deletion End | 158,625,145 |
| Deletion Size | 13,760 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 158,625,564 | 158,625,586 | GGCAGTAGGGTATTGAACACAAG |
| TUP | 158,625,588 | 158,625,611 | TCGTCAGCCTTGTGCTAGGAGCTA |
| TUR | 158,625,635 | 158,625,655 | AGACAGCCCATCCTCTGGAAG |
| TDF | 158,638,194 | 158,638,213 | CAAGCTGTGCAGGCCACTAC |
| TDP | 158,638,215 | 158,638,237 | TGCTCAGCCGTGTCCCAGACAAT |
| TDR | 158,638,261 | 158,638,280 | TCCAGGCCCTACCATGTTTC |
PCR Primers
| Primer | Sequence |
| SU | GATCTGCAAGGCTATGCAG |
| SD | AACAGTGCAAAGAGCAGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16080A-G11 | VGB6 | C57BL/6NTac | |
| 16080A-G10 | VGB6 | C57BL/6NTac | |
| 16080A-A5 | VGB6 | C57BL/6NTac | |
| 16080A-H9 | VGB6 | C57BL/6NTac | |
