| Gene | Wfdc8 |
| Chromosome | 2 |
| Deletion Start | 164,608,892 |
| Deletion End | 164,603,157 |
| Deletion Size | 5,736 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 164,608,545 | 164,608,562 | TGCGTCCACCCTGCTAAG |
| TUP | 164,608,519 | 164,608,544 | CCATTCTTCCACGGCAGTGCTCTTGG |
| TUR | 164,608,485 | 164,608,505 | CCTAACCCTCGGCTCTCATTG |
| TDF | 164,603,325 | 164,603,346 | ACTCATTCTTTGCTCCCTTACC |
| TDP | 164,603,304 | 164,603,324 | TCAGAGCCCTGCATGCTACCC |
| TDR | 164,603,281 | 164,603,300 | TCCTGGCAGTTTCCTTTGTC |
PCR Primers
| Primer | Sequence |
| SU | TGGAGCCAGAAGAAATCAGAAG |
| SD | TGTGAAGGCTTGCCAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16081B-G9 | VGB6 | C57BL/6NTac | |
| 16081B-E7 | VGB6 | C57BL/6NTac | |
| 16081B-D5 | VGB6 | C57BL/6NTac | |
| 16081C-B5 | VGB6 | C57BL/6NTac | |
| 16081C-G4 | VGB6 | C57BL/6NTac | |
| 16081C-E1 | VGB6 | C57BL/6NTac | |
