| Gene | Kcng1 |
| Chromosome | 2 |
| Deletion Start | 168,269,239 |
| Deletion End | 168,262,376 |
| Deletion Size | 6,864 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 168,269,209 | 168,269,229 | CGGGAGACAACTCGCACTATG |
| TUP | 168,269,184 | 168,269,206 | TACAGCGCTCTAAGCTGCGCCTC |
| TUR | 168,269,121 | 168,269,141 | CGGTAGAAGACGCCCTTGATG |
| TDF | 168,262,536 | 168,262,559 | GTCACCTCCATCTTCCATACTTTC |
| TDP | 168,262,513 | 168,262,535 | TCCCGCTCTTACCTGGAGCTCAA |
| TDR | 168,262,486 | 168,262,505 | CGGATCAGCACCCGTTCTTG |
PCR Primers
| Primer | Sequence |
| SU | TACATACTCCACAATCAGTG |
| SD | GTGCCCCAGCATATGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16082A-F10 | VGB6 | C57BL/6NTac | |
| 16082B-E2 | VGB6 | C57BL/6NTac | |
| 16082B-F11 | VGB6 | C57BL/6NTac | |
| 16082C-C7 | VGB6 | C57BL/6NTac | |
