| Gene | Zfp831 |
| Chromosome | 2 |
| Deletion Start | 174,657,805 |
| Deletion End | 174,643,537 |
| Deletion Size | 14,269 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 174,644,103 | 174,644,124 | GCGGACACAAACACATCTCAAC |
| TUP | 174,644,127 | 174,644,150 | CTCCCGCTTGTCCTTGGAGTCTGA |
| TUR | 174,644,154 | 174,644,172 | CAGAAGGCTGCTCCCACTC |
| TDF | 174,656,934 | 174,656,954 | TGCATACCGTGCGTTACAAAG |
| TDP | 174,656,957 | 174,656,982 | CGTGCACCAAGAGAATGCAGTCTCCA |
| TDR | 174,657,025 | 174,657,047 | ATGACCCAGATGGCAGACATCAC |
PCR Primers
| Primer | Sequence |
| SU | GTGTGTGTGGCCCTGTTG |
| SD | ACAATGATGGGATTGTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 114 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16083A-C8 | VGB6 | C57BL/6NTac | |
