| Gene | 9030418K01Rik |
| Chromosome | 2 |
| Deletion Start | 178,421,495 |
| Deletion End | 178,417,569 |
| Deletion Size | 3,927 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 178,417,619 | 178,417,641 | CGTGCCAAAGGTAAATCCTTCAG |
| TUP | 178,417,643 | 178,417,665 | TCTCCCGAGCGAGCTTCACTTGT |
| TUR | 178,417,679 | 178,417,699 | ACCCTGGCCTGCTCATTTCTG |
| TDF | 178,421,212 | 178,421,231 | CCAACGGCAACATCCGAATC |
| TDP | 178,421,232 | 178,421,254 | CCCAGGCAGTCTGCAGTGCTTCT |
| TDR | 178,421,260 | 178,421,279 | GGCCCTATGGGAATCCACTG |
PCR Primers
| Primer | Sequence |
| SU | AAGTAGGCATGACAGGCTGC |
| SD | GCCTCTCTTCCTCCACACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16084A-A8 | VGB6 | C57BL/6NTac | |
| 16084A-C7 | VGB6 | C57BL/6NTac | |
| 16084A-G4 | VGB6 | C57BL/6NTac | |
