| Gene | Cables2 |
| Chromosome | 2 |
| Deletion Start | 180,273,452 |
| Deletion End | 180,259,977 |
| Deletion Size | 13,476 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 180,272,782 | 180,272,802 | CCAGGACACAGGGTCAAGATG |
| TUP | 180,272,757 | 180,272,780 | CCAGCCCAAGATCAAGGTGTCGGG |
| TUR | 180,272,676 | 180,272,698 | GGCCCTCAAGTACCAGAATGAAG |
| TDF | 180,260,949 | 180,260,968 | TTGTAGCCAAGGGCAGTGTG |
| TDP | 180,260,925 | 180,260,947 | CCAAGCAGGACAGCCAGTCATGC |
| TDR | 180,260,822 | 180,260,844 | TGAGCATGTGGGACTTCAAAGAG |
PCR Primers
| Primer | Sequence |
| SU | CATAATGAACATTCGCTGAAG |
| SD | TCAAGGTGTCTGCCCTATCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 127 bp | + | + | - |
| TDF | TDR | 147 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16085A-A9 | VGB6 | C57BL/6NTac | |
| 16085A-B5 | VGB6 | C57BL/6NTac | |
| 16085A-B7 | VGB6 | C57BL/6NTac | |
| 16085A-B8 | VGB6 | C57BL/6NTac | |
| 16085A-D3 | VGB6 | C57BL/6NTac | |
