| Gene | Prpf6 |
| Chromosome | 2 |
| Deletion Start | 181,632,912 |
| Deletion End | 181,601,464 |
| Deletion Size | 31,449 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 181,602,132 | 181,602,155 | TTTCTCTAGGATGTGTGGCTGTAG |
| TUP | 181,602,157 | 181,602,184 | TCACCTGTTCAAATTCTCAGCCGTCTGG |
| TUR | 181,602,186 | 181,602,205 | TAGTGGCACTGCCATCTCTG |
| TDF | 181,632,818 | 181,632,836 | GCACAAGCTGTCCGTCATC |
| TDP | 181,632,837 | 181,632,865 | TCCCGCAGTCTGTTAGGATTTACATCAGA |
| TDR | 181,632,884 | 181,632,905 | CACGCTTCTTTGCTCGAATGTC |
PCR Primers
| Primer | Sequence |
| SU | AACAGGACTAATGCGTTCCG |
| SD | AGAGCACAGGCTATCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16086A-B10 | VGB6 | C57BL/6NTac | |
| 16086A-F12 | VGB6 | C57BL/6NTac | |
| 16086A-G4 | VGB6 | C57BL/6NTac | |
| 16086A-H6 | VGB6 | C57BL/6NTac | |
