| Gene | Cyb561d1 |
| Chromosome | 3 |
| Deletion Start | 108,200,771 |
| Deletion End | 108,199,192 |
| Deletion Size | 1,580 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 108,200,205 | 108,200,226 | ACAAACCACGGGACAGTATCAG |
| TUP | 108,200,183 | 108,200,204 | TGAAGCCCAGCCGTCAGCCTAT |
| TUR | 108,200,123 | 108,200,142 | ACCGCCAAGGCCATGAACAC |
| TDF | 108,199,413 | 108,199,431 | AAGGGTGGCTCGCCTCAAG |
| TDP | 108,199,388 | 108,199,411 | TCTACCATCTGACGTGTGGCCTGG |
| TDR | 108,199,348 | 108,199,367 | ATGCCCAAGAGCACCGTTAC |
PCR Primers
| Primer | Sequence |
| SU | ACTGGTTTCATGCTCTTGGG |
| SD | GCACTGCCTTCTCAATAGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16087A-G8 | VGB6 | C57BL/6NTac | |
| 16087B-G11 | VGB6 | C57BL/6NTac | |
