| Gene | Dcaf10 |
| Chromosome | 4 |
| Deletion Start | 45,374,297 |
| Deletion End | 45,342,171 |
| Deletion Size | 32,127 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,342,822 | 45,342,840 | GCCTGGCCTGTTGGGATAG |
| TUP | 45,342,842 | 45,342,863 | CAGGCCCAGTGCTTCCCTTCGG |
| TUR | 45,342,913 | 45,342,931 | TGTACTGGCTCCGACTGAC |
| TDF | 45,373,940 | 45,373,959 | CTCCAGTGCGACCAGTCTCC |
| TDP | 45,373,960 | 45,373,982 | CCTCGATGTTCCCTGCGGTTGAC |
| TDR | 45,373,993 | 45,374,013 | CCTGCCCACATTTGCTTCCTC |
PCR Primers
| Primer | Sequence |
| SU | GTAGATAATCCGACCCAG |
| SD | GAATGGTGGAGACAGGGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 110 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16092A-B4 | VGB6 | C57BL/6NTac | |
| 16092A-E3 | VGB6 | C57BL/6NTac | |
| 16092A-E6 | VGB6 | C57BL/6NTac | |
| 16092A-B9 | VGB6 | C57BL/6NTac | |
| 16092A-F3 | VGB6 | C57BL/6NTac | |
| 16092A-F9 | VGB6 | C57BL/6NTac | |
