| Gene | Hsdl2 |
| Chromosome | 4 |
| Deletion Start | 59,617,794 |
| Deletion End | 59,581,762 |
| Deletion Size | 36,033 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 59,582,292 | 59,582,313 | CACTCAGAAGCAGGGTCAGAAG |
| TUP | 59,582,324 | 59,582,350 | TCGAGGCTGTTCTGGTCTACATAGTGA |
| TUR | 59,582,365 | 59,582,385 | CTGTAGCGTTTGCTGACAGAG |
| TDF | 59,617,473 | 59,617,497 | TCAGGAAGTGAGATTCCCTAGATAG |
| TDP | 59,617,499 | 59,617,522 | AATATGAAGTGCTGTGTAGACGTC |
| TDR | 59,617,524 | 59,617,548 | GAGTCACATAAATTCCAGAGCACAG |
PCR Primers
| Primer | Sequence |
| SU | CAGCACCCTCAAGTCAGTCC |
| SD | CTGCAGTTCTTCACCCAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16094B-A6 | VGB6 | C57BL/6NTac | |
| 16094B-F10 | VGB6 | C57BL/6NTac | |
| 16094B-H3 | VGB6 | C57BL/6NTac | |
