| Gene | Repin1 |
| Chromosome | 6 |
| Deletion Start | 48,598,018 |
| Deletion End | 48,596,310 |
| Deletion Size | 1,709 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 48,596,655 | 48,596,674 | GGGCATAGCTTCCGAGGATG |
| TUP | 48,596,677 | 48,596,699 | TAGCCCTGGTTCTGCATCTGCGG |
| TUR | 48,596,723 | 48,596,742 | CGCATTCAGGGCAAGTGATG |
| TDF | 48,597,864 | 48,597,883 | GCCTTCTGTTGTGCCATCTG |
| TDP | 48,597,884 | 48,597,907 | TGGCCAGACCTTTGATGACGAGGA |
| TDR | 48,597,926 | 48,597,946 | TCTCAGGCATCATGCTTCTTC |
PCR Primers
| Primer | Sequence |
| SU | GTGCCAATTAGGGAGCTCTG |
| SD | GAACGGTTCTCTTCCCACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16114A-C11 | VGB6 | C57BL/6NTac | |
| 16114A-E6 | VGB6 | C57BL/6NTac | |
| 16114A-F1 | VGB6 | C57BL/6NTac | |
| 16114A-F8 | VGB6 | C57BL/6NTac | |
| 16114A-F9 | VGB6 | C57BL/6NTac | |
