16118   Scnn1a     Status: ES cell colonies screened / QC positives

GeneScnn1a
Chromosome6
Deletion Start125,344,131
Deletion End125,321,887
Deletion Size22,245
Genome Build38
MGI IDMGI:101782
NCBI Gene ID20276
BAC ID300F22
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 6

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF125,322,243125,322,265CGGCATGATGTACTGGCAGTTTG
TUP125,322,268125,322,296TTGCTGTTCGAGGAATACTTCAGCTACCC
TUR125,322,309125,322,331GCTTGTCCGAATTGAGGTTGATG
TDF125,343,672125,343,693GGGCATGGGTACTTCTTTCTTC
TDP125,343,696125,343,720CAGATGGTCAGCCTCCTGTCCAACC
TDR125,343,749125,343,769ACCACGGACAGCACAGATGAG

PCR Primers

PrimerSequence
SUCGGAGAGTCAAACAGTCC
SDTTGCCAAAGAGCTAACAAGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR89 bp++-
TDFTDR98 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
16118A-A1VGB6C57BL/6NTac
16118A-H1VGB6C57BL/6NTac
16118A-D1VGB6C57BL/6NTac
16118A-C9VGB6C57BL/6NTac
16118A-B9VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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