| Gene | Zfp592 |
| Chromosome | 7 |
| Deletion Start | 81,041,932 |
| Deletion End | 81,023,428 |
| Deletion Size | 18,505 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 81,023,474 | 81,023,491 | ATGTGCCAGCCGTGAGTG |
| TUP | 81,023,493 | 81,023,516 | CATTGTCAAGAACACCAGCCGCCA |
| TUR | 81,023,535 | 81,023,554 | TGGGAGCAATGTGGTCTTTC |
| TDF | 81,041,486 | 81,041,505 | GAGTTCCAGAGCCACATACC |
| TDP | 81,041,506 | 81,041,527 | TCAGCACCAGGTGGACAGCTCC |
| TDR | 81,041,538 | 81,041,558 | AGCACAAGCCACAGAGAAGAC |
PCR Primers
| Primer | Sequence |
| SU | AACTCAGGTATGTGACTTC |
| SD | AAGGGTGAACAAGGGACCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16125A-C6 | VGB6 | C57BL/6NTac | |
| 16125A-H11 | VGB6 | C57BL/6NTac | |
