16129   Ap2a2     Status: ES cell colonies screened / QC positives

GeneAp2a2
Chromosome7
Deletion Start141,597,611
Deletion End141,562,363
Deletion Size35,249
Genome Build38
MGI IDMGI:101920
NCBI Gene ID11772
BAC ID309P16
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 7

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Taqman primers not yet available.

PCR Primers

PrimerSequence
SUACAGCTCCGGTTCTCCAAG
SDGAAGGCACTGAGGCTGTG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
16129A-A10VGB6C57BL/6NTac
16129A-C7VGB6C57BL/6NTac
16129A-F9VGB6C57BL/6NTac
16129A-G10VGB6C57BL/6NTac
16129A-D2VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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