| Gene | 6330512M04Rik |
| Chromosome | 7 |
| Deletion Start | 142,371,097 |
| Deletion End | 142,350,414 |
| Deletion Size | 20,684 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 142,370,430 | 142,370,452 | AACCTCTTATCTTGGCCCATCTC |
| TUP | 142,370,399 | 142,370,425 | AGCTCATTTGCCAGGCACTGATACTCA |
| TUR | 142,370,362 | 142,370,383 | GTCATCAGGCTTCGGCATCTAC |
| TDF | 142,350,970 | 142,350,990 | TGAGAAGGCCCATGCTAAATC |
| TDP | 142,350,945 | 142,350,969 | TGAGACACATTCTGATGGCCGACCG |
| TDR | 142,350,882 | 142,350,902 | TGTCAGCAAGAGTGGGACAAG |
PCR Primers
| Primer | Sequence |
| SU | CACAGGGATTTCCATCTC |
| SD | CCAGACACACAAGGTTGTTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 109 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16130A-C2 | VGB6 | C57BL/6NTac | |
| 16130A-F6 | VGB6 | C57BL/6NTac | |
| 16130A-E5 | VGB6 | C57BL/6NTac | |
| 16130A-E11 | VGB6 | C57BL/6NTac | |
| 16130A-D7 | VGB6 | C57BL/6NTac | |
