| Gene | Osbpl5 |
| Chromosome | 7 |
| Deletion Start | 143,741,720 |
| Deletion End | 143,709,012 |
| Deletion Size | 32,709 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 143,741,293 | 143,741,316 | ACCTTTCTGTGTCCTACCTGATTC |
| TUP | 143,741,262 | 143,741,285 | TGGAGGAGGCAGGCTAAACTTCCT |
| TUR | 143,741,223 | 143,741,244 | AAGGGTCCTTCCTGTAGAGTTC |
| TDF | 143,709,611 | 143,709,632 | GGAGAACTATCGGCAGGAGAAG |
| TDP | 143,709,587 | 143,709,609 | AGCGGGCCACGAAGCAGCTGTTC |
| TDR | 143,709,549 | 143,709,567 | TCAGCCATGATGACCACAC |
PCR Primers
| Primer | Sequence |
| SU | TGCTATCATGCACAAGCCTC |
| SD | CCTATCCTGATCAGTTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16131B-F5 | VGB6 | C57BL/6NTac | |
| 16131B-H5 | VGB6 | C57BL/6NTac | |
| 16131C-G12 | VGB6 | C57BL/6NTac | |
| 16131C-G11 | VGB6 | C57BL/6NTac | |
| 16131C-F11 | VGB6 | C57BL/6NTac | |
