16141   Hsd11b2     Status: ES cell colonies screened / QC positives

GeneHsd11b2
Chromosome8
Deletion Start105,523,468
Deletion End105,518,868
Deletion Size4,601
Genome Build38
MGI IDMGI:104720
NCBI Gene ID15484
BAC ID311G19
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 8

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Taqman primers not yet available.

PCR Primers

PrimerSequence
SUGCGAAGCTGGCGACTGAC
SDTGGACTTGCAGCACTGAGAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
16141A-A11VGB6C57BL/6NTac
16141A-H5VGB6C57BL/6NTac
16141A-D9VGB6C57BL/6NTac
16141A-D12VGB6C57BL/6NTac
16141A-F9VGB6C57BL/6NTac
16141A-G4VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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