| Gene | Syce1l |
| Chromosome | 8 |
| Deletion Start | 113,654,991 |
| Deletion End | 113,643,240 |
| Deletion Size | 11,752 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 113,643,252 | 113,643,271 | GAGCCTTTGATGGCGGAGTG |
| TUP | 113,643,274 | 113,643,299 | TGGGAGCTGAGAAAGCTGAAGACACC |
| TUR | 113,643,307 | 113,643,326 | TCTACCCTGTTGGCCTTCAC |
| TDF | 113,654,386 | 113,654,406 | TGGTCCACCATCTCTGACTTG |
| TDP | 113,654,407 | 113,654,430 | TTGGCATCTGACCACCCATGTTGA |
| TDR | 113,654,437 | 113,654,456 | CCCGCATATCGTCCTCACTC |
PCR Primers
| Primer | Sequence |
| SU | GATGCTCTTAGCTGCCCAAG |
| SD | TGTGGCTCCCTCAGTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16142A-B1 | VGB6 | C57BL/6NTac | |
| 16142A-C3 | VGB6 | C57BL/6NTac | |
| 16142A-G2 | VGB6 | C57BL/6NTac | |
| 16142A-C8 | VGB6 | C57BL/6NTac | |
