| Gene | Raver1 |
| Chromosome | 9 |
| Deletion Start | 21,091,974 |
| Deletion End | 21,075,343 |
| Deletion Size | 16,632 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 21,091,477 | 21,091,498 | GGTTGAAGGTTGCAGGTAGTTG |
| TUP | 21,091,450 | 21,091,469 | CAGGCAGCTGTGGCCCTGCT |
| TUR | 21,091,390 | 21,091,409 | TGCATGGGTCAGTTGGAATC |
| TDF | 21,075,994 | 21,076,013 | ACTTCACCAGTGGCCTACAG |
| TDP | 21,075,970 | 21,075,992 | CTGGCCTCAAGCAGAGCCACCTG |
| TDR | 21,075,916 | 21,075,937 | CGGCATTCCAATGGTCCTAGAG |
PCR Primers
| Primer | Sequence |
| SU | GGATTCACGCCTGCCTAT |
| SD | CTCCAAAGGCACTCAAGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 98 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16143A-A7 | VGB6 | C57BL/6NTac | |
| 16143A-A10 | VGB6 | C57BL/6NTac | |
| 16143A-C12 | VGB6 | C57BL/6NTac | |
| 16143A-F10 | VGB6 | C57BL/6NTac | |
| 16143A-G7 | VGB6 | C57BL/6NTac | |
