| Gene | Mcart6 |
| Chromosome | X |
| Deletion Start | 137,015,342 |
| Deletion End | 136,982,569 |
| Deletion Size | 32,774 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 137,015,282 | 137,015,301 | GCTGTGGGAGCCATGTACTG |
| TUP | 137,015,256 | 137,015,279 | TGCTGTACCTGCTGGGTTACCAGG |
| TUR | 137,015,185 | 137,015,206 | CAGGGATGCGGGAACTAGAATC |
| TDF | 136,982,946 | 136,982,965 | CCCATTGGGTTCCTGCTTTG |
| TDP | 136,982,909 | 136,982,937 | TAGTGTCAATGGAACAATCACCTGCCTGA |
| TDR | 136,982,879 | 136,982,899 | TGGCAACTAGCACCATCAGAG |
PCR Primers
| Primer | Sequence |
| SU | GGAGTACAGCTGAGGCATCC |
| SD | TGGAAGTGAGAATGGCTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 117 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16156A-A3 | VGB6 | C57BL/6NTac | |
| 16156A-B10 | VGB6 | C57BL/6NTac | |
| 16156A-D7 | VGB6 | C57BL/6NTac | |
| 16156A-E9 | VGB6 | C57BL/6NTac | |
| 16156A-H3 | VGB6 | C57BL/6NTac | |
