| Gene | Bysl |
| Chromosome | 17 |
| Deletion Start | 47,611,168 |
| Deletion End | 47,601,553 |
| Deletion Size | 9,616 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 47,610,982 | 47,611,001 | TTTGCAGCAAGCCAGACAAC |
| TUP | 47,610,954 | 47,610,981 | AGCAGGAGGAGCTCGAAACTGACCATGG |
| TUR | 47,610,930 | 47,610,948 | GGTGGTGCTGACCGATCAC |
| TDF | 47,602,150 | 47,602,172 | TGGGCATCTAGGAAGAAAGTGTG |
| TDP | 47,602,118 | 47,602,143 | TGTGCGGATCGTGAGACATTTGGACA |
| TDR | 47,602,083 | 47,602,102 | GAGAGCAAGCAATCCCTGTG |
PCR Primers
| Primer | Sequence |
| SU | CTAGAACGTCATCGACTCG |
| SD | AGCAGTGACTCCCACCTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16205A-B5 | VGB6 | C57BL/6NTac | |
| 16205A-B7 | VGB6 | C57BL/6NTac | |
| 16205A-C3 | VGB6 | C57BL/6NTac | |
| 16205A-D2 | VGB6 | C57BL/6NTac | |
