| Gene | Foxp4 |
| Chromosome | 17 |
| Deletion Start | 47,904,514 |
| Deletion End | 47,867,314 |
| Deletion Size | 37,201 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 47,903,866 | 47,903,885 | GGTCCCTGAACCATGCTGTG |
| TUP | 47,903,836 | 47,903,862 | AGGTTGAACTTACAGGCACCAGTGGCC |
| TUR | 47,903,801 | 47,903,825 | TCCCTTCCTTTCCTAGTACCTCTTG |
| TDF | 47,867,943 | 47,867,962 | TCAGAGCGTGTGCATAGGTG |
| TDP | 47,867,916 | 47,867,941 | TCTCTGACCTGAACATCACAGTGCGG |
| TDR | 47,867,897 | 47,867,914 | GTTTCCAGCCGGGCTTTC |
PCR Primers
| Primer | Sequence |
| SU | CAATATGATGCTGTCTGCCC |
| SD | CATCACCAATCTGAGCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16206A-A7 | VGB6 | C57BL/6NTac | |
| 16206A-B1 | VGB6 | C57BL/6NTac | |
| 16206A-B9 | VGB6 | C57BL/6NTac | |
