| Gene | 1110038F14Rik |
| Chromosome | 15 |
| Deletion Start | 76,950,459 |
| Deletion End | 76,948,812 |
| Deletion Size | 1,648 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 76,948,995 | 76,949,014 | GCGCTGAAGGTATGGGAGTG |
| TUP | 76,949,018 | 76,949,038 | TCTGCGGGACCCTGTTACCCA |
| TUR | 76,949,055 | 76,949,072 | TCCGCCTGGGACAGAAAG |
| TDF | 76,950,279 | 76,950,300 | GTGAGGCCACCAGAAAGAAGAG |
| TDP | 76,950,304 | 76,950,325 | AAGGGTCTGCAGGCCTCGTCCT |
| TDR | 76,950,395 | 76,950,414 | ATGAGCGGTCCAGCATGTAG |
PCR Primers
| Primer | Sequence |
| SU | ATCCACCCAAGTGCTTTCTG |
| SD | GCCTCCTCTAACACATTCGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 136 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16218A-A6 | VGB6 | C57BL/6NTac | |
| 16218A-C4 | VGB6 | C57BL/6NTac | |
| 16218A-G6 | VGB6 | C57BL/6NTac | |
| 16218A-F1 | VGB6 | C57BL/6NTac | |
