| Gene | Phf13 |
| Chromosome | 4 |
| Deletion Start | 151,995,861 |
| Deletion End | 151,991,514 |
| Deletion Size | 4,348 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 151,994,962 | 151,994,980 | GCCTGGTGGAGCAAGTTGG |
| TUP | 151,994,936 | 151,994,960 | ACCGGGTTTGAAAGTGCTGCGTTGC |
| TUR | 151,994,911 | 151,994,929 | CCGAAAGCCTCCCGCAAAG |
| TDF | 151,992,059 | 151,992,078 | TGGACTCAGGTGAGGGATTG |
| TDP | 151,992,029 | 151,992,054 | CCTAGGGTCCAGGCCACTAGAGTTGG |
| TDR | 151,991,991 | 151,992,013 | TCCACACGTTCTCCTTCAAATAC |
PCR Primers
| Primer | Sequence |
| SU | CCGAGACACGAGCCGAAC |
| SD | CAACTTGGACGGCTGCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16229A-A2 | VGB6 | C57BL/6NTac | |
| 16229A-A8 | VGB6 | C57BL/6NTac | |
| 16229A-E2 | VGB6 | C57BL/6NTac | |
| 16229A-G10 | VGB6 | C57BL/6NTac | |
| 16229A-G11 | VGB6 | C57BL/6NTac | |
