| Gene | Wdr36 |
| Chromosome | 18 |
| Deletion Start | 32,866,226 |
| Deletion End | 32,837,391 |
| Deletion Size | 28,836 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,838,267 | 32,838,286 | TGAAAGCCTGGTGTGGTAAC |
| TUP | 32,838,296 | 32,838,323 | AGTGGGTTAGAATGTGCTTCTTCGCCCT |
| TUR | 32,838,334 | 32,838,353 | CAGGGAGGACGCATCAAGAC |
| TDF | 32,865,290 | 32,865,309 | GCACTGCATGGTGTGATGTG |
| TDP | 32,865,311 | 32,865,335 | TGAAGGCGTGGCTAACACTAGCTCA |
| TDR | 32,865,349 | 32,865,368 | GCCCGAGCTAGATGGAGAAC |
PCR Primers
| Primer | Sequence |
| SU | CCAGTTCTTGTCTCTTCCGC |
| SD | TCTCCCTGTGCAGACACTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16234A-F5 | VGB6 | C57BL/6NTac | |
| 16234C-E2 | VGB6 | C57BL/6NTac | |
