| Gene | 4933417A18Rik |
| Chromosome | 13 |
| Deletion Start | 34,953,100 |
| Deletion End | 34,924,540 |
| Deletion Size | 28,561 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,924,790 | 34,924,810 | TGACATTACAGGTGCCCTCAG |
| TUP | 34,924,812 | 34,924,837 | TCATGAGACGCTTGCCACATTATGGC |
| TUR | 34,924,872 | 34,924,892 | GACCTCAACTCATGGGCAAAG |
| TDF | 34,953,004 | 34,953,025 | CAAATAGTGCCAGGATGGTGAC |
| TDP | 34,953,026 | 34,953,047 | AGCGATTCCCACAGCTGGGCCA |
| TDR | 34,953,052 | 34,953,071 | GAAGCCTCTGGTTGCAGTAG |
PCR Primers
| Primer | Sequence |
| SU | TGTGCACACCAATCTCCTTC |
| SD | TGGTCCTGAACTCACTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16256A-A9 | VGB6 | C57BL/6NTac | |
| 16256A-F11 | VGB6 | C57BL/6NTac | |
| 16256A-C4 | VGB6 | C57BL/6NTac | |
| 16256A-F4 | VGB6 | C57BL/6NTac | |
| 16256A-H8 | VGB6 | C57BL/6NTac | |
