| Gene | Fam54a |
| Chromosome | 10 |
| Deletion Start | 20,360,638 |
| Deletion End | 20,347,914 |
| Deletion Size | 12,725 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,348,629 | 20,348,651 | CCAATGGGAGGAAGTTAGGACAG |
| TUP | 20,348,659 | 20,348,685 | AAACAAAGCAGGAACCTGGAGGCAGGA |
| TUR | 20,348,703 | 20,348,725 | GCTGGTGGAACAAGTCAGTTATC |
| TDF | 20,359,903 | 20,359,920 | CCTGGCAATCCAGCTCAC |
| TDP | 20,359,921 | 20,359,945 | TCTCATGCCTGGAGTCTGGTGTCTG |
| TDR | 20,359,952 | 20,359,971 | CAAGGCAGACAGTGGCTCAG |
PCR Primers
| Primer | Sequence |
| SU | GCTTGACACCTTACTGGCA |
| SD | AGGAGCCTCAGTAGGAAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16305A-D5 | VGB6 | C57BL/6NTac | |
| 16305E-A6 | VGB6 | C57BL/6NTac | |
