| Gene | Bdkrb2 |
| Chromosome | 12 |
| Deletion Start | 105,592,704 |
| Deletion End | 105,588,233 |
| Deletion Size | 4,472 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,588,491 | 105,588,511 | TGTGGGATGTCCCAGTCATTG |
| TUP | 105,588,512 | 105,588,537 | TGAAGACAGGGTCGCCATGGAATTGA |
| TUR | 105,588,554 | 105,588,574 | CCAAGGCTTCCCTTCGTCTTC |
| TDF | 105,592,438 | 105,592,458 | GTCATCACGCAGATCAGTTCC |
| TDP | 105,592,459 | 105,592,481 | TACGTGGCCTACAGCAACAGCGG |
| TDR | 105,592,492 | 105,592,511 | CCCACGATCACGTACACCAG |
PCR Primers
| Primer | Sequence |
| SU | TCACCCTCATGGAGAATG |
| SD | TTGAAACTGGGAAATGAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16334A-A10 | VGB6 | C57BL/6NTac | |
| 16334A-B9 | VGB6 | C57BL/6NTac | |
| 16334A-G12 | VGB6 | C57BL/6NTac | |
| 16334A-D7 | VGB6 | C57BL/6NTac | |
| 16334A-D11 | VGB6 | C57BL/6NTac | |
| 16334A-D3 | VGB6 | C57BL/6NTac | |
