| Gene | Ddx42 |
| Chromosome | 11 |
| Deletion Start | 106,248,177 |
| Deletion End | 106,224,101 |
| Deletion Size | 24,077 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 106,224,656 | 106,224,675 | CCTCCTTTCGAGGGTCACAG |
| TUP | 106,224,676 | 106,224,700 | ACATGTGCCAACAAACCCTGCTGGG |
| TUR | 106,224,728 | 106,224,748 | CCTTCACTAGCCCACGCTTTC |
| TDF | 106,248,064 | 106,248,085 | TAGCAGCAGGATGGACAAAGTG |
| TDP | 106,248,087 | 106,248,115 | ACAGCAAGACAGATAAGACACCTGATGGC |
| TDR | 106,248,120 | 106,248,139 | TTTGGGTGGCTCTGGAACAG |
PCR Primers
| Primer | Sequence |
| SU | TTGTCTTGCAATTTCGATCC |
| SD | CCTAACAGGGCAGAAGTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16335A-A8 | VGB6 | C57BL/6NTac | |
| 16335A-A12 | VGB6 | C57BL/6NTac | |
| 16335A-B8 | VGB6 | C57BL/6NTac | |
| 16335A-C11 | VGB6 | C57BL/6NTac | |
| 16335A-G7 | VGB6 | C57BL/6NTac | |
| 16335A-D2 | VGB6 | C57BL/6NTac | |
