| Gene | Hnrnpa2b1 |
| Chromosome | 6 |
| Deletion Start | 51,469,673 |
| Deletion End | 51,463,381 |
| Deletion Size | 6,293 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 51,469,137 | 51,469,160 | GACGTGTTTAGCGATTTTCCTTTC |
| TUP | 51,469,106 | 51,469,133 | TGGAAGTCTTCATACCGTTTCGAGGTGG |
| TUR | 51,469,071 | 51,469,090 | CAGGACGCTGAAGACCCAAG |
| TDF | 51,464,040 | 51,464,063 | GGTGATTGGGAAGTCATGGAAGTC |
| TDP | 51,464,010 | 51,464,036 | ACAGTTCTGCATCTCAGAAAGCGAGGA |
| TDR | 51,463,964 | 51,463,986 | TTAACAGTTACACGAGGACACTC |
PCR Primers
| Primer | Sequence |
| SU | ACGGCGTTATTTCAAACTGC |
| SD | TCTGCATCTGCTCTGGTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16361A-A6 | VGB6 | C57BL/6NTac | |
| 16361A-B10 | VGB6 | C57BL/6NTac | |
| 16361A-B12 | VGB6 | C57BL/6NTac | |
| 16361A-G10 | VGB6 | C57BL/6NTac | |
| 16361A-D8 | VGB6 | C57BL/6NTac | |
| 16361A-D3 | VGB6 | C57BL/6NTac | |
