| Gene | Gypc |
| Chromosome | 18 |
| Deletion Start | 32,559,822 |
| Deletion End | 32,529,941 |
| Deletion Size | 29,882 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,559,520 | 32,559,540 | CAAGTCAAGTCAAGCGGTCAG |
| TUP | 32,559,496 | 32,559,517 | TCAACTTCGCACCAGGGAGCCC |
| TUR | 32,559,445 | 32,559,463 | GGGCCAGAAAGAGCTGTTG |
| TDF | 32,530,168 | 32,530,186 | TGGCCTTGGTCCTGGTCTG |
| TDP | 32,530,140 | 32,530,167 | CCTCCTCTTTCTCATGCTTCGCTACCTG |
| TDR | 32,530,093 | 32,530,113 | TCTGTGCCTTTGGCCTCATTG |
PCR Primers
| Primer | Sequence |
| SU | TCTGATAGTGGCAGATGG |
| SD | TCGTCTTGAGCTGCATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16375A-F1 | VGB6 | C57BL/6NTac | |
| 16375C-B11 | VGB6 | C57BL/6NTac | |
