| Gene | Syt14 |
| Chromosome | 1 |
| Deletion Start | 193,035,632 |
| Deletion End | 193,026,976 |
| Deletion Size | 8,657 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 193,035,117 | 193,035,137 | ACCGAGATGCTGAGGTGAATG |
| TUP | 193,035,087 | 193,035,111 | CTGAGGTATTGAGAAGCGCCGTGCT |
| TUR | 193,035,007 | 193,035,029 | GTGGTTATCATCGGCCCAGAAAG |
| TDF | 193,027,716 | 193,027,739 | GGCAGTGCTTATGAAGATGAGTAG |
| TDP | 193,027,682 | 193,027,709 | AATTGGTGGCTAACTGCTATGTCTGGCC |
| TDR | 193,027,655 | 193,027,678 | TCAGCTATCACAACACATGCAAAC |
PCR Primers
| Primer | Sequence |
| SU | GTTAAGTCGAGGTTGTGGGC |
| SD | CCTCTGTTCTCTGCCTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 131 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16376B-H4 | VGB6 | C57BL/6NTac | |
| 16376C-B3 | VGB6 | C57BL/6NTac | |
| 16376C-D3 | VGB6 | C57BL/6NTac | |
| 16376C-D7 | VGB6 | C57BL/6NTac | |
| 16376C-C12 | VGB6 | C57BL/6NTac | |
| 16376C-G2 | VGB6 | C57BL/6NTac | |
