| Gene | Apba1 |
| Chromosome | 19 |
| Deletion Start | 23,917,694 |
| Deletion End | 23,892,809 |
| Deletion Size | 24,886 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,893,564 | 23,893,582 | CGAGAAGGAGGCGGAGTTC |
| TUP | 23,893,586 | 23,893,608 | CCCTACCCGCGCATGGACAGTTA |
| TUR | 23,893,622 | 23,893,641 | GCCACGATCTGGTCGATGTC |
| TDF | 23,917,291 | 23,917,312 | GCCAAGTGCCAAACAGACAAAC |
| TDP | 23,917,314 | 23,917,338 | TTGAACAGACTGTCTGAGCCTCCCA |
| TDR | 23,917,347 | 23,917,369 | TCTCTTAGCCAGACCTGTAATCC |
PCR Primers
| Primer | Sequence |
| SU | CTCTATCCATAGACCTCG |
| SD | GGTGACTGTGTTCAAACTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16413A-B9 | VGB6 | C57BL/6NTac | |
| 16413A-C3 | VGB6 | C57BL/6NTac | |
