| Gene | Pdcd5 |
| Chromosome | 7 |
| Deletion Start | 35,647,368 |
| Deletion End | 35,642,116 |
| Deletion Size | 5,253 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 35,646,873 | 35,646,894 | GGACTCCAGAGATGGTGCTCAG |
| TUP | 35,646,854 | 35,646,872 | CCCCTGCGGTGCCGGCTAG |
| TUR | 35,646,819 | 35,646,840 | TGTCCCAAGGCAAGTTTACAAC |
| TDF | 35,642,567 | 35,642,585 | TGGGAGGTGGTAGCGTTTC |
| TDP | 35,642,547 | 35,642,566 | CAGCTGCCTGAGCTGGCCAA |
| TDR | 35,642,461 | 35,642,484 | GGTAGGTGTGAGAGGTAAGAAGTG |
PCR Primers
| Primer | Sequence |
| SU | TTGCCAGTCCTAGGAATC |
| SD | AATCCAAAGCTGGGACTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 125 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16429A-B1 | VGB6 | C57BL/6NTac | |
| 16429A-B4 | VGB6 | C57BL/6NTac | |
| 16429A-C2 | VGB6 | C57BL/6NTac | |
| 16429A-D1 | VGB6 | C57BL/6NTac | |
| 16429A-H4 | VGB6 | C57BL/6NTac | |
| 16429A-F4 | VGB6 | C57BL/6NTac | |
