| Gene | Myl10 |
| Chromosome | 5 |
| Deletion Start | 136,700,968 |
| Deletion End | 136,693,386 |
| Deletion Size | 7,583 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 136,694,208 | 136,694,226 | GCGGTTCAGAGCCAATGTC |
| TUP | 136,694,228 | 136,694,254 | CCATCAGGCCTTCACTATCATGGACCA |
| TUR | 136,694,297 | 136,694,316 | ACTCACCCAGTGCAGCAAAG |
| TDF | 136,700,788 | 136,700,811 | AACATCCATTGTTCTGACTTCTCC |
| TDP | 136,700,818 | 136,700,846 | CAACAGTCCCTATGTAACCCTCAACTCCC |
| TDR | 136,700,866 | 136,700,883 | GGCGGGAAAGCTGCAAAC |
PCR Primers
| Primer | Sequence |
| SU | CAAGGATACAGCCCAGCTTC |
| SD | CTCACGACCTGCACATGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16431A-A11 | VGB6 | C57BL/6NTac | |
| 16431A-B3 | VGB6 | C57BL/6NTac | |
| 16431A-D11 | VGB6 | C57BL/6NTac | |
| 16431A-E2 | VGB6 | C57BL/6NTac | |
