| Gene | Lman1l |
| Chromosome | 9 |
| Deletion Start | 57,620,667 |
| Deletion End | 57,607,099 |
| Deletion Size | 13,569 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 57,619,878 | 57,619,900 | GGAGATGGAAGCGGATATGAGAG |
| TUP | 57,619,850 | 57,619,875 | TCTTAGCCCACAGGTCTGTGCTCTGG |
| TUR | 57,619,814 | 57,619,833 | GGTGAACAGGATCGGACAAG |
| TDF | 57,607,227 | 57,607,245 | TGTCCACAGGAAGCCTTTC |
| TDP | 57,607,201 | 57,607,226 | TCTAGAGCCTGCATTACCCATCACCA |
| TDR | 57,607,169 | 57,607,189 | GATGGGCTGTCTCCTCAGAAC |
PCR Primers
| Primer | Sequence |
| SU | CCTGGCACTAAGCATTCCTG |
| SD | GGGACTTGTCCTTGTCCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16457A-A6 | VGB6 | C57BL/6NTac | |
| 16457A-C6 | VGB6 | C57BL/6NTac | |
| 16457A-E11 | VGB6 | C57BL/6NTac | |
| 16457A-G2 | VGB6 | C57BL/6NTac | |
