| Gene | Zfyve21 |
| Chromosome | 12 |
| Deletion Start | 111,827,912 |
| Deletion End | 111,814,309 |
| Deletion Size | 13,604 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 111,814,651 | 111,814,670 | ACGGAGGAACGGTGCCAAAG |
| TUP | 111,814,683 | 111,814,712 | TCGAAGTTCTAAACTCTGCAAAGCTTGGCC |
| TUR | 111,814,731 | 111,814,750 | GCATGGAATGCGTGAATTGC |
| TDF | 111,827,062 | 111,827,082 | GGGACGATCTGCTTGGCTTAC |
| TDP | 111,827,090 | 111,827,110 | TGGCCAGGCCATTCCGAAAGC |
| TDR | 111,827,112 | 111,827,130 | GCAGTGGAGGCACAGAAAC |
PCR Primers
| Primer | Sequence |
| SU | GAGTCTGCTCCACCCAGAAG |
| SD | AGAAAGCTGACGCCTTCCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16480A-B4 | VGB6 | C57BL/6NTac | |
| 16480A-B7 | VGB6 | C57BL/6NTac | |
| 16480A-D6 | VGB6 | C57BL/6NTac | |
| 16480A-D12 | VGB6 | C57BL/6NTac | |
| 16480A-B2 | VGB6 | C57BL/6NTac | |
