| Gene | Mgrn1 |
| Chromosome | 16 |
| Deletion Start | 4,916,150 |
| Deletion End | 4,886,389 |
| Deletion Size | 29,762 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,886,833 | 4,886,852 | GGATTTGGACCCAGGCTTTC |
| TUP | 4,886,853 | 4,886,875 | CTCTTCCCGTGCGAGTGAGGGAC |
| TUR | 4,886,903 | 4,886,922 | GAACCCAGCACCTCCCTATC |
| TDF | 4,915,214 | 4,915,232 | CGTCTGCCTCTGCCTTCTG |
| TDP | 4,915,235 | 4,915,258 | TGCTGGGATTAAAGACGAGCGCCA |
| TDR | 4,915,260 | 4,915,278 | TCCAGTTTAGCCGGGTGTG |
PCR Primers
| Primer | Sequence |
| SU | TTCCGGTTTCAGTATCCAGC |
| SD | CCTATCTGGACTACAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16482A-B9 | VGB6 | C57BL/6NTac | |
| 16482A-A4 | VGB6 | C57BL/6NTac | |
| 16482A-C12 | VGB6 | C57BL/6NTac | |
| 16482A-H12 | VGB6 | C57BL/6NTac | |
