| Gene | 5430416O09Rik |
| Chromosome | 4 |
| Deletion Start | 43,731,445 |
| Deletion End | 43,731,050 |
| Deletion Size | 396 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,731,076 | 43,731,096 | AGCTCCAGACGTAGCTTCACC |
| TUP | 43,731,103 | 43,731,124 | ACGTTTCACCAGGAGGCGTCGC |
| TUR | 43,731,140 | 43,731,159 | CAGTGGCCGGGATTGGAGAG |
| TDF | 43,731,255 | 43,731,273 | GCAGTGTCTGGTGTCTCAC |
| TDP | 43,731,275 | 43,731,298 | CCTCCCAGCTCTCCATCCGTATCC |
| TDR | 43,731,311 | 43,731,331 | TGGAGGGAGGCAAGAAGTTTG |
PCR Primers
| Primer | Sequence |
| SU | GACCACAGCTCTTGGAGGTC |
| SD | TTGTGCTCTCTGTGGCTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16526A-B6 | VGB6 | C57BL/6NTac | |
| 16526A-H12 | VGB6 | C57BL/6NTac | |
