| Gene | 4933411G11Rik |
| Chromosome | 5 |
| Deletion Start | 143,204,654 |
| Deletion End | 143,192,301 |
| Deletion Size | 12,354 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 143,192,589 | 143,192,610 | CAAGGTAGAGGGAGGACTGGTG |
| TUP | 143,192,614 | 143,192,641 | TTGCTCAATGGGTAAAGGCACCTGTTGC |
| TUR | 143,192,653 | 143,192,674 | GCCGCAAGGATCAATCTGAAAC |
| TDF | 143,203,892 | 143,203,912 | TTCACAGGGACAACAACCAAG |
| TDP | 143,203,917 | 143,203,942 | AAGAAGGGTCACAAGCCAAGGGTAGC |
| TDR | 143,203,944 | 143,203,967 | GCAGACAAGTGCTATATTCCACAG |
PCR Primers
| Primer | Sequence |
| SU | CATCTAGCGCACCATAGG |
| SD | CAATTCTCACTTACATGAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16533A-F8 | VGB6 | C57BL/6NTac | |
| 16533A-E2 | VGB6 | C57BL/6NTac | |
