| Gene | Cyp2ab1 |
| Chromosome | 16 |
| Deletion Start | 20,313,889 |
| Deletion End | 20,309,746 |
| Deletion Size | 4,144 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,313,101 | 20,313,121 | GTGGTGATCGACCTGTTTCTG |
| TUP | 20,313,079 | 20,313,098 | AGGCACAGACACCACGGCCA |
| TUR | 20,313,026 | 20,313,046 | ACCTTGGATGGCTCTGTGATG |
| TDF | 20,310,229 | 20,310,252 | CCTCAAACTACTTCAGCTCTTCTC |
| TDP | 20,310,200 | 20,310,226 | CAGGGAACCATCATCCTGCCCAATCTG |
| TDR | 20,310,170 | 20,310,191 | CCAGCACTCAGGGTCATACAAC |
PCR Primers
| Primer | Sequence |
| SU | TTTGGCCATCATTTCCTCTC |
| SD | CCTTCTGGTCTGAAGATGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16542A-A3 | VGB6 | C57BL/6NTac | |
| 16542A-A8 | VGB6 | C57BL/6NTac | |
| 16542A-C5 | VGB6 | C57BL/6NTac | |
| 16542A-D1 | VGB6 | C57BL/6NTac | |
| 16542A-D11 | VGB6 | C57BL/6NTac | |
| 16542A-E4 | VGB6 | C57BL/6NTac | |
