| Gene | Pin1-ps1 |
| Chromosome | 2 |
| Deletion Start | 104,714,782 |
| Deletion End | 104,714,255 |
| Deletion Size | 528 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 104,714,740 | 104,714,763 | CCGGCTGGAAGAAGTATATGAGTC |
| TUP | 104,714,713 | 104,714,738 | CAGCTCAGGCCGGGAATACTACTTCA |
| TUR | 104,714,693 | 104,714,710 | GCTGGCGTTGGTGATGTG |
| TDF | 104,714,387 | 104,714,407 | GAGAAACCGTTTGAGGATGCA |
| TDP | 104,714,362 | 104,714,386 | TCGTTTGCTCTACGGACTGGGGAGA |
| TDR | 104,714,332 | 104,714,350 | TGCCTGACTCCGTGAACAC |
PCR Primers
| Primer | Sequence |
| SU | AAGTGAACCATCTGGGAATTG |
| SD | TGCATCTTGAGGCTGAGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16549B-D11 | VGB6 | C57BL/6NTac | |
| 16549B-D12 | VGB6 | C57BL/6NTac | |
| 16549B-G10 | VGB6 | C57BL/6NTac | |
