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Sequence Downloads and Strategy Information
Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 59,074,636 | 59,074,658 | GCTCAATGGTCCTATTCCTCTTC |
| TUP | 59,074,609 | 59,074,634 | TGACTCAGCTCTCCAGGAAGTTGGTG |
| TUR | 59,074,572 | 59,074,591 | CCACCTGGCTCAGTCACAAG |
| TDF | 59,051,071 | 59,051,091 | GGCGTCCTCTGTGAGAGTAAC |
| TDP | 59,051,044 | 59,051,069 | CATGCTCTTCGCTGCTGAGCTAACTC |
| TDR | 59,051,015 | 59,051,037 | TCCAGACACAATCCCTTTCAGTG |
PCR Primers
| Primer | Sequence |
| SU | GCCTTAGCAGAACCGCATAC |
| SD | ACACATGCTGTTTACTGAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the KOMP Repository.| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16555A-A3 | VGB6 | C57BL/6NTac | |
| 16555A-G11 | VGB6 | C57BL/6NTac |
