| Gene | Zfp212 |
| Chromosome | 6 |
| Deletion Start | 47,931,660 |
| Deletion End | 47,920,729 |
| Deletion Size | 10,932 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 47,921,629 | 47,921,649 | TGTTGAGACAGAGCCTCACAG |
| TUP | 47,921,652 | 47,921,680 | TGTCCTTAAGCTGATGACAATCTTCCCGA |
| TUR | 47,921,708 | 47,921,728 | CCCAGCAGAATGACTTACACC |
| TDF | 47,931,411 | 47,931,428 | GCACCAGCGCATTCACAC |
| TDP | 47,931,432 | 47,931,454 | TGAGCGACCCTACAGTTGCCCTG |
| TDR | 47,931,460 | 47,931,483 | CTGCTTCTGGACAAAGCTCTTCTC |
PCR Primers
| Primer | Sequence |
| SU | TCTTAAAGCATTTCGCGGAG |
| SD | AGCACATACCTCTGTGGCCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16592A-A2 | VGB6 | C57BL/6NTac | |
| 16592A-D6 | VGB6 | C57BL/6NTac | |
| 16592A-D12 | VGB6 | C57BL/6NTac | |
| 16592A-G7 | VGB6 | C57BL/6NTac | |
