16654   Hexdc     Status: ES cell colonies screened / QC positives

GeneHexdc
Chromosome11
Deletion Start121,222,436
Deletion End121,207,695
Deletion Size14,742
Genome Build38
MGI IDMGI:3605542
NCBI Gene ID238023
BAC ID414I17
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 11

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF121,208,523121,208,547GGATGCTGGAAAGATAGCTCAGTAG
TUP121,208,554121,208,577TGTTTGCCACCAGGCCTAATGACC
TUR121,208,595121,208,617TCTTGCCCACCATAAGACAAGTC
TDF121,221,781121,221,802TCTAAGTGGTTCCTGGCCTAAG
TDP121,221,807121,221,831TATCAACCACATGCCCAAGCTGACC
TDR121,221,836121,221,855CAGGTGGCCTGATTCCAAAG

PCR Primers

PrimerSequence
SUGACAGGTGGGAAAGGTGT
SDTGCCACTAGTTCTGACTTGGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR95 bp++-
TDFTDR75 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
16654A-A9VGB6C57BL/6NTac
16654A-A11VGB6C57BL/6NTac
16654A-B9VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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