| Gene | Ly6g |
| Chromosome | 15 |
| Deletion Start | 75,158,742 |
| Deletion End | 75,155,648 |
| Deletion Size | 3,095 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GCGAAACCATCATCACATACCAG |
| TUP | | | AGTCTCTTGGACCTCACTACCTGTGCCC |
| TUR | | | GTGCAGCAGTGTAGAATCTAAGG |
| TDF | | | GCAGACTCTCACAGAAGCAAAG |
| TDP | | | CAAGAGCAATCTCTGCCTTCCCATCTGC |
| TDR | | | TTCACGTTGACAGCATTACCAG |
PCR Primers
| Primer | Sequence |
| SU | AAAGACCTTGTCACTGTGCC |
| SD | TATGTGTGGATGGTGATGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16678A-C4 | VGB6 | C57BL/6NTac | |
| 16678A-H6 | VGB6 | C57BL/6NTac | |
| 16678A-H7 | VGB6 | C57BL/6NTac | |
