| Gene | Vps25 |
| Chromosome | 11 |
| Deletion Start | 101,259,118 |
| Deletion End | 101,253,758 |
| Deletion Size | 5,361 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 101,254,106 | 101,254,126 | GCACAAACAGTCCAGCATGAC |
| TUP | 101,254,129 | 101,254,150 | TGATGGAAGCCCAGGAGAGCCC |
| TUR | 101,254,166 | 101,254,186 | GGGAGGATACGCTGTAGCTTG |
| TDF | 101,258,962 | 101,258,980 | GCAAGAGCACAAGGCTGAG |
| TDP | 101,258,983 | 101,259,006 | TATCACTGTCAGCGATGGCCGAGG |
| TDR | 101,259,034 | 101,259,057 | GGTGGGAGGTAAGAAGTAAAGGAG |
PCR Primers
| Primer | Sequence |
| SU | GATTCTAACTTGCGTTCGGC |
| SD | GGCAACAGCAGCTAAAGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16685A-C3 | VGB6 | C57BL/6NTac | |
| 16685A-C5 | VGB6 | C57BL/6NTac | |
| 16685A-D3 | VGB6 | C57BL/6NTac | |
| 16685A-G3 | VGB6 | C57BL/6NTac | |
