| Gene | Cdh26 |
| Chromosome | 2 |
| Deletion Start | 178,472,448 |
| Deletion End | 178,441,405 |
| Deletion Size | 31,044 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 178,441,417 | 178,441,438 | CGATCCAAGAGAAGATGGGTTC |
| TUP | 178,441,439 | 178,441,461 | TCACCACCCTGGAACTGCAGGAG |
| TUR | 178,441,537 | 178,441,556 | TTCCAAGGAGTCCTCTCCAG |
| TDF | 178,472,342 | 178,472,360 | CGGTGCTCCTGCACATTTG |
| TDP | 178,472,362 | 178,472,387 | ACCCAAGAGGCTCAGAGGCTTCATCC |
| TDR | 178,472,417 | 178,472,441 | GGACTTTGCTTTCCTCGTTATACAC |
PCR Primers
| Primer | Sequence |
| SU | TTTGTGCAAAGTCCTAAAGCC |
| SD | AACACAAATGCCTCTGCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 140 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16686A-A2 | VGB6 | C57BL/6NTac | |
| 16686A-B11 | VGB6 | C57BL/6NTac | |
| 16686A-E10 | VGB6 | C57BL/6NTac | |
| 16686A-F8 | VGB6 | C57BL/6NTac | |
| 16686A-H11 | VGB6 | C57BL/6NTac | |
