| Gene | Brp44 |
| Chromosome | 1 |
| Deletion Start | 165,481,000 |
| Deletion End | 165,461,406 |
| Deletion Size | 19,595 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 165,461,501 | 165,461,521 | CCACCCGGCAGGTAATGAATC |
| TUP | 165,461,522 | 165,461,544 | CTGAACGGCTCGCACGGAGATCG |
| TUR | 165,461,558 | 165,461,578 | GGCCTCTAATGTCCCGGTTTC |
| TDF | 165,480,369 | 165,480,391 | AGGAATGGTGTGGCTGTTAGGAG |
| TDP | 165,480,392 | 165,480,416 | TGCCAGTGGCTCTTTCTGAGGACTG |
| TDR | 165,480,452 | 165,480,470 | TTGGAGTTACAGGCGGTTG |
PCR Primers
| Primer | Sequence |
| SU | AGCACCTGTTGCCATGACTC |
| SD | GTATGCATGACCAAGTACGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 16687B-E11 | VGB6 | C57BL/6NTac | |
| 16687C-C11 | VGB6 | C57BL/6NTac | |
| 16687C-G3 | VGB6 | C57BL/6NTac | |
| 16687C-H6 | VGB6 | C57BL/6NTac | |
